20th-banner-rev.png

Albany 2019: 20th Conversation - Abstracts

category image Albany 2019
Conversation 20
June 11-15 2019
Adenine Press (2019)

Effect of concentration of ligands on DNA-biosensor average output signal and dispersion

Nowadays DNA-biosensors are intensively applied for resolution of actual bio-analytical and medical problems, such as identification of biological material by primary sequence of nucleotides, improvement of early diagnostic and treatment methods of oncological diseases, determination of pharmacological preparations of anti-cancerous action (Kavita, 2017). Application of DNA-biosensors significantly decreases both analysis cost and time of its carrying out as compared to traditional medico-biological methods. It is connected to the fact that DNA-biosensors have small sizes and demand relevantly less amounts of the studying samples. Meanwhile, the decrease of DNA-biosensor sizes inevitably results in both falling down of its output signal value and increasing of level of the signal fluctuations. It should be mentioned that DNA-biosensor output signal fluctuations may be conditioned by arbitrary character of the binding of small molecules (ligands) in the solution with DNA duplexes on the biosensor as well. By this reason the studies of the effect of ligands on average value of DNA-biosensor output signal and its fluctuations become actual and important. In this work in the framework of relativistic approach the dependences of DNA-biosensor output signal average value and its dispersion on ligand concentration in the solution were theoretically calculated. The characteristic peculiarities of DNA-biosensor output signal were studied. It was shown that the output signal of DNA-biosensors monotonously increases with ligand concentration enhancement in the solution (Arakelyan et al, 2006). It was also shown that the output signal relaxation time decreases along with ligand concentration increasing in the solution. Calculations showed that the dispersion dependence of DNA-biosensor output signal on the ligand concentration in the solution has a form of a curve with maxima. From the beginning with concentration increasing of ligands in the solution DNA-biosensor output signal dispersion increases, then passing through the maximum it decreases and tends to zero at the further enhancement of ligand concentration. The obtained results can be used in the practice at preparation of micro DNA-biosensors and analysis of the measurement data.

References

Arakelyan, V.B., Babayan, S.Yu., Tairyan, V.I., Arakelyan, A.V., Parsadanyan, M.A.& Vardevanyan P.O. (2006). Kinetics of ligand binding to nucleic acids. J. Biomol. Str. Dyn. 23, 479-484. Kavita, V. (2017). DNA Biosensors-A Review. J.Bioengineer.& Biomedical Sci.7, 222. doi:10.4172/2155-9538.1000222

Valeri B. Arakelyan1
Marine A.Parsadanyan2
Poghos O. Vardevanyan2*

1 Department of Molecular Physics
2 Department of Biophysics
Yerevan State University
Yerevan, Armenia

Ph: (+37460) 710522
Email: p.vardevanyan@ysu.am