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Albany 2019: 20th Conversation - Abstracts

category image Albany 2019
Conversation 20
June 11-15 2019
Adenine Press (2019)

Cisplatin and Estradiol Joint Action on Quantities of Rat Brain Chromatin Phospholipids

Nowadays it is well known that nuclear lipids, including the chromatin bound ones, are important for regulating many essential cellular processes such as DNA replication, transcription and gene expression. Recent advances demonstrated the involvement of nuclear lipids in remodeling of chromatin and epigenetic regulation of gene expression. Furthermore it was shown that lipids of chromatin can regulate the chromatin structure via alteration of some enzymes activity, which accomplished histone modifications as well as inverse processes [Kuvichkin, 2016].

Our previous results showed the reliable changes in phospholipids in rat brain chromatin preparations after the in vivo action of cisplatin. It is impossible to exclude the significance of chromatin lipids quantitative alterations for cisplatin antitumor effects realization. Unfortunately the using of this drug is greatly limited because of its severe side effects. The results of recently investigations established that combination of steroid hormone estradiol and cisplatin in chemotherapy treatment schemes decreased cisplatin induced toxicities [Ghasemi et al., 2016].

Taking intо foregoing consideration it may be interesting to explore the cisplatin and estradiol joint in vivo action on total quantities of rat brain chromatin phospholipids as well as changes of their individual fractions content.

Оur results revealed reliably decrease (by 24 %) of the total amount of phospholipids from rat brain chromatin preparations after treatment with the antitumor drug cisplatin whereas the combinated injection of cisplatin and estradiol restored the baseline level. Joint use of cisplatin and estradiol lead to recovery the compliance rate of phosphatidylcholine and phosphatidylethanolamine content. Unlike this the quantity of sphingomyelin and phosphatidylinositol was increased in comparison to baseline as well as cisplatin treatment variants. Cisplatin and estradiol combinated treatment provides significant effect on cardiolipin content. The absolute quantity of this phospholipid reduced by 38% instead of 15% in case of cisplatin alone treatment.

Changes in content of different phospholipids fractions caused by cisplatin in vivo action may play a definite role in nuclear lipid metabolic pathways. These changes should be considered particular negative side effects during the basic antitumor effect of cisplatin. However, the changes of phospholipids in case of cisplatin and estradiol combinated treatment may be helpful for reducing of cisplatin toxicity and eliminating of its side effects.

References

    V.V. Kuvichkin DNA-Lipids-Me2+ Complexes Structure and their Possible Functions in a Cell (2016). J Chem Biol Ther 1(1),1-6.

    M.Ghasemi, M. Nematbakhsh, Z. Pezeshki, N. Soltani, M. Moeini, A. Talebi (2016). Nephroprotective effect of estrogen and progesterone combination on cisplatin-induced nephrotoxicity in ovariectomized female rats.Indian J Nephrol.26(3), 167-175.

Nune Hakobyan
Zhenya Yavroyan
Agapi G. Hovhannisyan
Emil S. Gevorgyan

Department of Biophysics
Yerevan State University
Yerevan, Armenia

Ph:(374) 55 03 43 36
Email: nhakobyan@ysu.am