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Albany 2019: 20th Conversation - Abstracts

category image Albany 2019
Conversation 20
June 11-15 2019
Adenine Press (2019)

Changes in the stability and conductivity of BLM in the presence of zinc oxide nanoparticles

Currently, nanoparticles are intensively implanted in almost all areas of scientific and practical human activity, although there are fears that they may damage biological systems (L.K. Limbach et al, 2005).The effect of nanoparticles beginning with their primary interaction with biological membranes. However, since biological membranes display a very complex composition in terms of lipids and proteins, it seems appropriate to investigate the effect of nanoparticles on a bilayer lipid membrane (BLM), which mimics well the lipid bilayer of a biological membrane. This work is devoted to the study of the effect of zinc oxide nanoparticles (ZnO), with an average size of 50 nm, on the stability and conductivity of BLM. The experiments were performed on BLM obtained from a mixture of 1,2-dipalmitoyl-sn-glycero-3-[phospho-1-serine](DPPS) and 1,2-dipalmitoyl-sn-glycero-3-phospho¬cho¬line (DPPC) of the company Avanti Polar Lipids in a ratio 1:1. Voltage, in the range of 0.20-0.55 V, was applied to the BLM using chlorine-silver electrodes connected to the ADC and controlled by a computer. Experimental points were obtained depicting the dependence of the average BLM lifetime on the potential in the absence and presence of zinc oxide nanoparticles in solution. The parameters characterizing the BLM stability were determined from fitting the experimental data with a theoretical curve for the dependence of the average lifetime of the BLM on the potential (V.F. Pastushenko et al, 1979). This is the BLM tension, the linear tension of the pore edge in the BLM, as well as the parameter which is the product of the number of pores on the BLM and the pore diffusion coefficient in radius space. It was shown that ZnO nanoparticles increase BLM stability in an electric field. With an increase in the concentration of nanoparticles, the stability of BLM also increases. It was shown that ZnO nanoparticles increase the value of the linear tension coefficient of the pore edge, which is formed in BLM, which also leads to an increase in the stability of BLM in an electric field. It was also shown that ZnO nanoparticles lead to a decrease in the conductivity of BLM.

References

    L.K. Limbach, R.N.Grass, T.J. Brunner, M.A. Hintermann, M. Muller, D. Gunter, W.J. Stark. (2005). Oxide Nanoparticle Uptake in Human Lung Fibroblasts: Effects of Particle Size, Agglomeration, and Diffusion at Low Concentrations. Environ. Sci.Technol. v.39, p.9370-9376.

    V.F. Pastushenko, Yu.A. Chizmadzev, V.B. Arakelyan. (1979). Electric Breakdown of Bilayer Lipid Membranes II. Calculation of the membrane lifetime in the steady-state diffusion approximation. Bioelectrochemistry and Bioenergetics, v. 6, p 37-52.

Anahit L. Torosyan
Gayane V. Ananyan
Valeri B.Arakelyan*

Department of Molecular Physics
Yerevan State University
1 Alex Manoogian St.
0025, Yerevan, Armenia

Email:v.arakelyan@ysu.am