Issue August 2010
Volume 28No. 1 (1-132) August 2010 ISSN 0739-110 Peculiarities of Interaction of Porphyrins with tRNA at Low Ionic Strength (123-131)The interaction of meso-tetra-(4N-oxyethylpyridyl)porphyrin (TOEPyP4) and its Zn(II)-, Cu(II)-, Mn(III)-derivatives with tRNA from E.Coli at low ionic strength (µ=0.02M) was studied using UV/Vis spectrophotometry and Circular Dichroism (CD) methods.
An unusual Induced Circular Dichroism (ICD) spectra profile of the ZnTOEPyP4-tRNA complex is found. It is demonstrated that ZnTOEPyP4 is ordered in a stack, not only on helical sites, but also on loops of a hairpin form of tRNA. TOEPyP4 and CuTOEPyP4 are able to intercalate in the helical sites of this form of tRNA. MnTOEPyP4 interacts with tRNA via external non-ordered mechanism. It is established that all porphyrins are bound with tRNA more strongly than with DNA.
Y. Dalyan1,* 1Yerevan State University, Faculty
of Physics, Department of Molecular Physics, 1, A.Manoogian St., 0025,
Yerevan, Armenia Subscription is more cost effective than purchasing PDFs on-the-fly. Click here for details. |
|
Member Login | Home | Subscribe | Cancer Watch | Cancer Technologies | Editorial Officers | Instructions | Editor-in-Chief | Contact Us
Adenine Press, 2066 Central Avenue, Schenectady, NY 12304 USA |
Re-initialize IP-based Login |
