Issue December 2002
Volume 20No. 3 (p 311-486) December 2002 ISSN 0739-1102 Revealing of the Spin-Spin Interaction Due to Incorporating a Spin Label in the Carbohydrate Parts of Immuniglobulins M and G (p. 389-396)The EPR spectra of the preparations produced by spin labeling of the carbohydrate parts in monoclonal IgM and normal IgG with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl as the spin label indicate the existence of a rapid spin-spin exchange interaction between two spin labels. In the case of spin-labeled IgM, the carrier of such a spectrum is shown to be a glycopeptide noncovalently bound to IgM; it includes two spin labels and may be detached from the macromolecule by a combination of dialysis and gel filtration.
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