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Book of Abstracts: Albany 2009

category image Albany 2009
Conversation 16
June 16-20 2009
© Adenine Press (2008)

Binding of Some Antitumour Compounds with the DNA-radiated Millimeter Electromagnetic Waves

In water-salt solution the molecules of water form definite spatial structures the reverberatory absorption frequency of which is the millimeter range of electromagnetic waves. In the given work the thermodynamic parameters of the binding of intercalating compounds of mitoxantrone, ametantrone and nonintercalating-nitropsin with the calf thymus DNA, previously radiated by the millimeter coherent electromagnetic waves of nonthermal intensity, are studied. The water solutions of the DNA, prepared for the spectrophotometric titration, were radiated for 90 minutes. The DNA solutions were radiated at resonant (64,5 and 50,3 GHz) and nonresonant 48,3 GHz for water structures frequencies. The VHF generators were applied for radiation. The density of the stream power at the sample was ˜50 microwatt/sm2. The experiments show that both for the radiated nonradiated DNA the same pattern of the changes of DNA solution absorption which is a results of the binding with antitumour compounds is observed. Consequently, under the investigated conditions they interact both with the radiated and nonradiated DNA in the same means. The binding constant (K) and the stoichiometry of antitumour compounds with the radiated DNA are calculated from the stoichiometry titration spectrum. Using the value of (K) it is possible to define the changes of Gibbs free energy and enthropy at the binding of investegated compounds with the radiated DNA. Calculations show that they form a more stable complex (K inereases) with the DNA ratiated at resonant for water structures 64,5 and 50,3 GHz frequencies. This leads to more significant changes of the complexes enthropy. When the same DNA solutions are radiated at 48,3 GHz frequency, the thermodynamic parameters of antitumour compounds binding with the radiated DNA change insignificantly as compared with nonradiated DNA and are within the range of experiment error. Consequently, as a result of DNA radiation at resonant for water structures frequencies, such changes in the hydrate shell of the DNA occur, that antitumour compounds form more stable complex with them.

Yu. S. Babayan
G.L. Kanaryan
S. Yu. Babayan
P.S. Khazaryan
L.R. Grigoryan

Yerevan State Medical Univ.
2 Korjun Str.
Yerevan 0025
Armenia

ysbaba@ysmu.am