Book of Abstracts: Albany 2009
June 16-20 2009
© Adenine Press (2008)
Base Sequence and the Architecture of Nucleosomal DNA
In order to understand the mechanisms by which DNA base sequence and tightly bound proteins control the biophysical properties of the long, threadlike molecule, we have developed a coarse-grained model, in which the DNA base pairs are treated as rigid bodies subject to realistic, knowledge-based energy constraints, and computational techniques to determine the configuration-dependent propensities of these molecules. The presentation will highlight some of the unique, sequence-dependent spatial information that has been gleaned from analyses of the high-resolution structures of DNA and its complexes with other molecules, including nucleosomes, and illustrate how this information can be used to gain new insights into the positioning of non-specific proteins on DNA.
Wilma K. Olson
Department of Chemistry & Chemical Biology