Book of Abstracts: Albany 2007
June 19-23 2007
Application of Differential Pulse Voltammetry Revealing the Peculiarities of EtBr-DNA Complexes
The unique fluorescence characteristics of EtBr have provided a convenient way to study of interaction with DNA and, therefore, the application of other methods seems to be neglected. Meanwhile, the investigation of the EtBr interaction of DNA with the electrochemical method, which is versatile sensitive and applicable to most of the organic and inorganic species, may be successfully used for revealing the peculiarities of EtBr-DNA complex formation.
In this study, we used differential pulse voltammetry (DPV) method to obtain information on the interaction of EtBr with double-stranded DNA (dsDNA). Based on the relationship derived for the interaction study we reveal two binding types of EtBr with DNA. The binding constant (K) and the binding site size (n) of the interactions were obtained at two different ionic strength of solution, which agrees very well with the reported in the literature data. It, thus, provides another approach for the interaction studies of DNA to other electro activity dye molecules in addition to the spectrophotometric methods conventionally used.
Poghos O. Vardevanyan1
1Department of Biophysics