Book of Abstracts: Albany 2007
June 19-23 2007
Addressable Molecular Node Assembly -- A Generic Platform of Nano-scale Functionalised Surfaces Based on a ?Digitally Addressable? Molecular Grid
DNA is a powerful molecule for design of nano-networks because of its inherent rigidity and highly selective base-pairing, combined with the ability to conveniently produce any base sequence. Recently, a variety of repeating DNA-networks built up from complex motifs, with a unit size of approximately 100 nm, as well as smaller DNA constructs formed by simple duplexes, with a unit size of down to ∼20 nm, have been created and characterized. We here report a strategy by which oligonucleotides can be assembled into networks whose fundamental cells will be the smallest yet reported (diameter ∼10 nm) and whose structure, in contrast to other constructs, can be made non-repeating and, hence, addressable in terms of their DNA-base code. Using linear DNA as well as newly developed three-way branched (trigonal) oligonucleotide building-blocks to make a non-repeating DNA-nano-network with the highest realisable information density, we here demonstrate the formation of its fundamental cell, a DNA hexagon with an edge length of 4 or 8 nm. A straightforward solid-phase synthesis of trigonal oligonucleotides, developed using branching phosphoramidite monomers, allows full freedom in sequence and directionality of each arm providing the basis for a new bottom-up design of DNA-nano-networks. Together with the small unit-size of the network, these properties will allow functionalization with sub-nanometer precision, e.g., using triplex-forming oligonucleotides to selectively address a specific position in this network. This yields unprecedented richness in information density, important in context of Moore?s Law, and great potential for future nano-chip technology.
John Tumpane1, *
1Department of Chemical and Biological Engineering/Physical Chemistry