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Book of Abstracts: Albany 2011

category image Albany 2011
Conversation 17
June 14-18 2011
©Adenine Press (2010)

A Controlled DNA Biped Walker on a DX Track

Molecular machines perform elegant work with high efficiency in biological systems. They have inspired attempts to create artificial machines that mimic the ability to produce controlled motion (1, 2). We describe the construction of an extendable DNA walker-track system. A monomer DX track with three foot-holders is constructed, and the biped walker moves along the cylinder side of the track. Therefore the terminus of the monomer track could be used to create sticky ends for track extension and the total steps the walker could take increases as more monomer tracks are added. A dimer DX track with six foot-holders demonstrates the feasibility of track extension. By means of sequential addition of DNA set/unset strands (3, 4), the walker is programmed to move forward and then backward along the track. PAGE analysis demonstrates that the walker-track complex forms well. Psoralen cross-link monitoring is performed with an aliquot of material at each step. The PAGE analysis results further establish the step-wise formation of the expected products.



The schematic above shows the attachment of walker to the monomer DX track via a set strand (a), and the attachment of walker to a dimer DX track (b).

References

  1. M. Fennimore, T. D. Yuzvinsky, Wei-Qiang Han, M. S. Fuhrer, J. Cumings, A. Zettl, Nature 424, 408-410 (2003).
  2. N. C. Seeman, Nature 421, 427-431 (2003).
  3. W. B. Sherman and N. C. Seeman, Nano Lett.4, 1203-1207 (2004).
  4. J. Shin and N. A. Pierce, J. Am. Chem. Soc. 126, 10834-10835 (2004).

Dadong Li
Ruojie Sha
James Canary
Nadrian C. Seeman

Department of Chemistry
New York University
New York, NY 10003, USA

Ph: (212) 998-8395
Fx: (212) 260-7905
ned.seeman@nyu.edu